Nicholas Narwold

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Questions related from Nicholas Narwold

PCR primers bind to specific DNA at specific temperatures but not other temperatures?

I ran PCR in order to analyze a P-element excision on a mutant drosophila line. My results were not conclusive, however that is beside the point. I ran a temperature gradient in order to optimize...

26 January 2021 9,280 11 View

Does using ethanol for de-waxing drosophila heads before dissection interfere with future protein extraction or fly brain imaging?

I have heard that dipping fly heads in 70% ethanol before placing in cold PBS for dissection can help strip the heads of wax and allow them to stay submerged in the PBS during dissection. I was...

11 August 2020 4,445 2 View