Nicholas A Wallace

3 Questions 11 Answers 0 Followers

Questions related from Nicholas A Wallace

Will DNA readily transfer from PDMS to glass coverslips?

I've been using glass coverslips to minimize the volume of solutions that I need for washes, etc of my PDMS chips with combed DNA. Since I began this modification, I've noticed that the DNA...

15 June 2015 9,701 4 View

Does low pH affect yoyo binding or fluorescence?

Hi, I am developing an assay based around seeing yoyo stained DNA after a one hour treatment with 1M HCl. The DNA is "naked" (ie, stripped of all proteins) and stretched on a PDMS gel. Does anyone...

03 June 2015 7,589 3 View

Is anyone familiar with instances where RAD51 foci persist in cells following IR?

We are trying to determine the cause of the phenotype we have observed, but other than RAD54, we do not really have good ideas of where and what to look at.

13 June 2013 7,225 8 View