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Questions related from Nicholas Clay
I have been attempting to recreate an organotypic skin culture using primary fibroblasts and primary keratinocytes. I start by embedding fibroblasts in a collagen gel at 200,000-300,000 cells/mL....
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I have an amide-containing compound, and I would like to show that it is undergoing hydrogen bonding with a polar aprotic solvent. Can this be done with FT-IR?
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Tween vs. Triton-X---is there any preference for staining membrane proteins?
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