Nicholas Ankenbruck

2 Questions 2 Answers 0 Followers

Questions related from Nicholas Ankenbruck

Has anyone seen random errors appear in plasmid DNA sequences after assembly with the NEB HiFi kit?

I'm using a multistep approach to insert multiple genes in sequence into the pHIV lentiviral vector. I am PCR amplifying the genes from either gblocks from IDT or other plasmids. I use PCR to...

21 April 2021 5,625 1 View

Help figuring out why only lifted cells express eGFP after transduction?

I'm attempting to transduce HEK293T cells with a lentivirus expressing eGFP. I have been observing a low transduction efficiency and after a 24 h transduction the only cells expressing eGFP are...

04 September 2020 9,668 5 View