What is the best way to use CloneJET PCR cloning kit from Thermo for Successful ligation and transformation in E. coli cells?

i have been trying to blunt and Ligate my GOI into the PJET1.2 blunt and transform it in E. coli strain by following the protocol provided by Thermo but no colonies were obtained after transformation

11 August 2023 9,698 2 View