What can I do to prevent NS1 protein precipitation during refolding?

I am currently refolding the NS1 Dengue protein in a refolding buffer of PBS 1X pH 7.2 + 30% sucrose (protein stabilizer). I dissolved the inclusions with 1X PBS buffer pH 7.2 + 8M Urea + 30%...

25 April 2024 6,618 1 View