Nghiem Le

5 Questions 5 Answers 0 Followers

Questions related from Nghiem Le

Wrong p53 size on Western blot?

Hi I am blotting for p53 in my experiment. The predicted MW is 53 kDa. However the actual MW appears to be higher than the expected MW in SDS-PAGE. I loaded 10 micro-gram protein. I didn't have...

12 July 2017 7,540 4 View

Identify cysteine residue that is responsible for disulfide bond formation?

Hi all, I am doing a small project in which I see that a mutant in protein A, that changes arginine to cysteine, can form a disulfide bond with another cysteine protein B. The problem is that...

10 May 2016 2,054 3 View

Antibody against pro-caspase 3 also recognizes same position of beta-actin?

Hi all, I am testing the anti-apoptotics effects of a miRNA on H9c2 cell lines, in specific I focus on capsase 3. After 48 hours tranfecting H9c2 cells with mimic and antagomir, I collected cell...

29 February 2016 7,516 3 View

Can anyone tell me how to identify targeted proteins pertaining to apoptosis pathway?

Hi everyone, I am doing a project to predict and prioritize miRNAs and their targets that are involved in regulating apoptosis. We have already had the results of miRNAs expression and their...

01 February 2016 2,753 3 View

Is anyone familiar with postranscriptional regulation?

Can any body help me explain whether the following statement is true or false? If the site of transcript cleavage and polyadenylation of a particular RNA in one cell is downstream of the same...

05 October 2014 5,416 9 View