Nghiem Le

8 Questions 9 Answers 0 Followers

Questions related from Nghiem Le

Wrong p53 size on Western blot?

Hi I am blotting for p53 in my experiment. The predicted MW is 53 kDa. However the actual MW appears to be higher than the expected MW in SDS-PAGE. I loaded 10 micro-gram protein. I didn't have...

12 July 2017 7,556 4 View

Protocol for siRNA expression plamid?

Hi all, our lab is studying the role of a microRNA in cardioprotection. We want to observe what will happen if we remove this microRNA. We've tried to tranfect cells with antagomir/lipofectamin...

08 January 2017 3,215 4 View

Duration of oxygen glucose deprivation (OGD) for H9c2 cell line?

Hi all, I am appreciated if someone could suggest the duration for oxygen glucose deprivation (OGD) to mimic the ischemia/reperfusion injury for H9c2 cell line. Thanks,

18 August 2016 3,976 3 View

Identify cysteine residue that is responsible for disulfide bond formation?

Hi all, I am doing a small project in which I see that a mutant in protein A, that changes arginine to cysteine, can form a disulfide bond with another cysteine protein B. The problem is that...

10 May 2016 2,064 3 View

Antibody against pro-caspase 3 also recognizes same position of beta-actin?

Hi all, I am testing the anti-apoptotics effects of a miRNA on H9c2 cell lines, in specific I focus on capsase 3. After 48 hours tranfecting H9c2 cells with mimic and antagomir, I collected cell...

29 February 2016 7,528 3 View

Can anyone tell me how to identify targeted proteins pertaining to apoptosis pathway?

Hi everyone, I am doing a project to predict and prioritize miRNAs and their targets that are involved in regulating apoptosis. We have already had the results of miRNAs expression and their...

01 February 2016 2,762 3 View

How do I count the number of rabbit cardiomyocytes?

I do appreciate if any one could suggest any way to count the number of cell/ml because I want to have equal amount of rabbit myocytes in order to infect them with adenovirus. Thanks

18 May 2015 5,853 6 View

Is anyone familiar with postranscriptional regulation?

Can any body help me explain whether the following statement is true or false? If the site of transcript cleavage and polyadenylation of a particular RNA in one cell is downstream of the same...

05 October 2014 5,424 9 View