Ng Yee Ling

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Questions related from Ng Yee Ling

Ideal peptide fractionation (2D) compatible for iTRAQ labeled peptides?

May I know anyone of you that are working on iTRAQ, what kind of peptide fractionation method / brands you used or recommend for prior to nanoLC (eksigent) separations other than OFFGEL? Anyone of...

24 October 2016 5,269 3 View

Detailed/modified protocols for membrane proteins before iTRAQ labeling due to difficulty in resolubilizing?

I am working on membrane proteins from entamoeba histolytica. Im using membrane protein extraction kit from Calbiochem, and I did acetone precipitation because the protein profiles are better when...

21 October 2016 8,284 2 View