Neslihan Ögel

4 Questions 3 Answers 0 Followers

Questions related from Neslihan Ögel

How and why show different databanks different miRNA-numbers and targets for a specific gene?

Hi everyone, I am trying to analyze the miRNA-Targets of the FOSL1 gene but I am facing a particular problem. With Targetscan 7.2: -> 2 different miRNA Targets for FOSL1 got predicted. The...

02 February 2019 8,474 3 View

How can I find out which regulatory elements the 3' UTR of a specific gene contains ?

I used CRISPR/Cas9 to cut the FOSL1 gene within the last Exon and 3' UTR. RT-qPCR and Western Blot Analysis revealed downregulation of mRNA and protein. How can I find out which exact regulatory...

02 February 2019 3,911 3 View

Which protein concentration is suitable for WB with FOSL1-antibody (FRA1- antibody)?

Hey guys, Since I don't have time or capacities for testing the FOSL1-antibody (also known as FRA-1) I wanted to ask those of you who already worked with this antibody. Could anybody recommend...

10 October 2018 9,510 1 View

What is the efficiency of Cas9 crispr knockout in tetraploid organisms?

Hi guys, I tried a CRIRPS mediated knockout on HEK-cells and unfortunately it wasn't successful. Any ideas what could be the reason? There are several points which would come to my mind, like...

08 August 2018 8,377 0 View