Nelly Minarve

2 Questions 3 Answers 0 Followers

Questions related from Nelly Minarve

Ramos cells not growing after GFP+ cell sort?

Hi everyone, I have been transfecting Ramos cells with my gRNA construct using an amaxa nucleofector and I proceed by sorting GFP+ cells (one cell per well) into 96 well plates. I also include a...

28 September 2024 386 3 View

Digested vector is not showing any bands on gel?

I digested my cas9 vector by adding 5ug of vector, 3ul of NEB 2.1 buffer, 3ul of BbsI enzyme and completed to 30ul with ultra-pure H2O. I incubated overnight at 37C and added 1 ul of CIAP...

08 March 2024 1,154 3 View