Digested vector is not showing any bands on gel?

I digested my cas9 vector by adding 5ug of vector, 3ul of NEB 2.1 buffer, 3ul of BbsI enzyme and completed to 30ul with ultra-pure H2O. I incubated overnight at 37C and added 1 ul of CIAP...

08 March 2024 1,122 3 View