Nehal Aasem Saif

2 Questions 2 Answers 0 Followers

Questions related from Nehal Aasem Saif

How can EDTA be removed from already extracted DNA samples?

Our lab has extracted bacterial DNA samples using Qiagen DNeasy kit and eluted the samples in AE buffer. However, this buffer contains 0.5mM EDTA not suitable for downstream application. Any ideas...

14 July 2024 1,630 3 View

How to perform a proper Bacterial RNA extraction using limited resources?

I am trying to extract RNA from bacterial cells (E.coli) grown in M9 till they reach OD 0.5. Problems i have encountered: 1. Culture takes 6-7 hours to reach OD (M9 content "1 L" =100 ml 10xM9...

18 July 2018 2,451 3 View