Neha vinod Ahuja

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Questions related from Neha vinod Ahuja

During hypoxia treatment we are not able to see HIF1A stabilization in breast cancer cells? What could have gone wrong?

HIF1A protein gets stabilized after some time, but we are not able to see the band (stabilization) on western blot after various time points.

12 December 2016 8,511 6 View

Why platinum taq amplification of a gene is giving smear on the gel?

i am amplifying 6.7kb fragment using HiFi platinum taq. conditions used are: annealing temperature 72 deg celsius gDNA 600ng MgSO4 1.5mM But i am getting smear again and again. Kindly suggest how...

07 July 2016 6,492 1 View