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I know the application of IPTG in blue-white screening and recombinant protein expression. Does IPTG have any application in mammalian cell culture as growth enhancer or suppressor or any other...
28 October 2017 4,157 0 View
I am trying to ligate 4818 bp vector with 4 different inserts (3kb, 3.5 kb, 5.3kb and 6.5 kb) separately which would yield me 4 different products. the vector and inserts are cut with two...
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Is it possible to ligate a fragment of size 2.3 kb cut with XhoI and SacII into a vector which has RE site for XhoI only?
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