Neha Saini

4 Questions 5 Answers 0 Followers

Questions related from Neha Saini

Does IPTG have any application in mammalian cell culture related to growth etc. except recombinant protein expression?

I know the application of IPTG in blue-white screening and recombinant protein expression. Does IPTG have any application in mammalian cell culture as growth enhancer or suppressor or any other...

28 October 2017 4,183 0 View

Isolation of smooth muscle cells from human umbilical artery?

I am trying to isolate smooth muscle cells from human umbilical artery. I am using collagenase and elastin but I am getting mixture of smooth muscle and endothelial cells. Can anyone suggest a...

09 November 2016 3,436 6 View

Why do I get numerous colonies after transformation?

I am trying to ligate 4818 bp vector with 4 different inserts (3kb, 3.5 kb, 5.3kb and 6.5 kb) separately which would yield me 4 different products. the vector and inserts are cut with two...

24 April 2015 3,560 5 View

How do you ligate a fragment cut with XhoI and SacII, into a vector cut with XhoI only?

Is it possible to ligate a fragment of size 2.3 kb cut with XhoI and SacII into a vector which has RE site for XhoI only?

02 March 2015 3,239 4 View