Neha Khandelwal

2 Questions 4 Answers 0 Followers

Questions related from Neha Khandelwal

How to know which cell line/tissue has the high copy number of the various genes?

I have to amplify my gene sequences (IRF4, EBF1) from the genome. I thought of using HEK293T cells but am still unsure if this has a high copy number for these genes. How to check which cell line...

04 February 2022 7,906 2 View

How to determine transcriptional activity by Luciferase assay?

I have selected two transcription factors whose activity I want to measure by luciferase reporter assay. For this do I have to prepare two constructs, a: Luciferase gene with a transcription...

06 January 2022 2,195 3 View