Neha Kachewar

2 Questions 6 Answers 0 Followers

Questions related from Neha Kachewar

Has anyone done an in-vitro ubiquitination assay with E.coli lysate having recombinant E3 but other components (E1, E2, Ub) being purified protein?

E1, E2, biotinylated Ubiquitin and the buffer are from Enzolife sciences ubiquitination kit ( BML-UW9920-0001 ). I want to check whether my protein of interest, which is a E3 from rice, has...

02 July 2018 9,056 2 View

Can anyone help with a blank problem in Luminol based chemiluminescence for detection of ROS in Arabidopsis leaves and seedlings?

I am using Luminol based chemiluminescence for detection of ROS in Arabidopsis leaves and seedlings. Blank (Luminol+HRP+KOH, without any tissue), is generating luminescence higher than the...

09 August 2015 3,614 6 View