Neha Gupta

3 Questions 3 Answers 0 Followers

Questions related from Neha Gupta

How is the molecular weight of protein the western blot reported as 126kDa, the antibody catalogue and literature says its 164kDa-180kDa approx?

I am a research scholar working on this protein called pregnancy zone protein (PZP). I was facing problem in getting western blot bands of the same, so I checked the literature and found this...

05 June 2025 9,472 1 View

How can I precipitate a protein from condition media ?

So I want to see is the level of a secreted protein in the condition media via western blotting What my doubt is 1. How much cells should I seed and what should be the volume of condition...

20 June 2024 2,046 1 View

What should be the ideal semi dry transfer conditions (volt/mili ampere/time period) for detection of proteins ranges from 240-250kDa by western blot?

I want to transfer a 245kDA protein . I am using 8% gel and PVDF membrane. What are the semi dry conditions that i should use to achieve a complete transfer

14 June 2023 2,891 5 View