Neha Gupta

2 Questions 3 Answers 0 Followers

Questions related from Neha Gupta

How can I precipitate a protein from condition media ?

So I want to see is the level of a secreted protein in the condition media via western blotting What my doubt is 1. How much cells should I seed and what should be the volume of condition...

20 June 2024 1,814 1 View

What should be the ideal semi dry transfer conditions (volt/mili ampere/time period) for detection of proteins ranges from 240-250kDa by western blot?

I want to transfer a 245kDA protein . I am using 8% gel and PVDF membrane. What are the semi dry conditions that i should use to achieve a complete transfer

14 June 2023 2,811 5 View