Neethu Kamarudheen

5 Questions 8 Answers 0 Followers

Questions related from Neethu Kamarudheen

How to avoid starch precipitation from growth media?

My actinobacteria grow in 2.5% soluble starch supplemented to the media. After I obtain the supernatant and store at 4 degree Celsius for a couple of days, a white mass which I believe to be...

25 October 2017 6,704 3 View

How can the interference of the color of my test compound be nullified in a UV-spec assay?

The actinobacterial test compound under study is slightly colored yellow. This seems to disturb the result of various assay performed at various wavelengths. At a wavelength of 405 where...

24 February 2017 637 3 View

What could be the probable reason for a layer of dense sticky layer at the interface of solvents during organic solvent extraction?

On performing organic solvent-solvent extraction with cell free supernatant of actinomycetes, I observe a thick sticky layer between the two liquids. What could this be indicating?

13 August 2016 7,479 4 View

Can L-tyrosine be used in place of L-DOPA for melanin estimation?

In the process of confirmation and estimation of melanin pigments from actinomycetes can L-Tyrosine be used instead of L-tyrosine. The organism shows good diffusion of brown pigments in ISP 6 and...

23 March 2016 8,568 2 View

Does Casein dissolve the best in NaOH?

For protease inhibitor assay with casein as a substrate what shall I use for hammarsten casein to dissolve completely?

26 February 2016 6,888 5 View