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Questions related from Neeraj K Satija
I am aware of Streptavidin magnetic beads which can be used with biotinylated antibody in MACS. But I would like to know if anyone has tried isolating or depleting cells from heterogeneous...
06 June 2016 8,714 2 View
I am growing mESCs in 2i medium. I have cryopreserved mESCs cultured in DMEM+FBS in 90%FBS+10% DMSO but would like to know how to cryopreserve cells grown in 2i medium which is free of serum. I...
03 March 2016 4,091 5 View
I would like to know if it will be correct to perform normalisation of raw microarray data on every pair of samples to be compared (eg, control vs treatment 1, control vs treatment 2, etc) and...
11 November 2013 611 6 View
I have been trying to detect APC (adenomatous polyposis coli) from RIPA lysates of HEK and SaOS2 cells by western. However, I am not able to detect the protein (Mol Wt ~300kDa). I have tried...
03 March 2013 8,225 8 View
Does anyone have experience of transfecting siRNA in hMSC?
05 May 2012 4,540 0 View
I am facing a problem with all cell lines and primary cells I am using like hMSCs, SaOS2, HEK, NIH3T3. Since past about a week, the cells have started to detach from the surface and this...
05 May 2012 1,998 92 View
Hello everyone, I am trying to optimise MOI for transducing hMSCs using TurboGFP lentiviral particles (from Sigma). I incubated cells for 16h at MOI 1,5 & 10, and analysed after 48h by flow...
06 June 2010 5,370 2 View
