10 Questions 32 Answers 0 Followers
Questions related from Neelofar Mirza
Hi, I am trying to map my illumina PE reads on two Rhizoctonia solani genome,AG1-IA and AG1-IB. I have downloaded the fasta files for AG1-IA genome from RSIADB...
14 December 2016 1,524 3 View
Hello, I need annotation (.gtf or .gff) file for Oryza sativa indica (not japonica) for transcriptome assembly and annotation. I tried using the indica(93-11) annotation file from BGI-RIS FTP...
17 August 2016 7,685 3 View
Hello, I need the gene description file for rice (japonica and indica both) for my RNASeq annotation work. 1. can anyone direct me to the source? 2. What is the file format for such a file?
17 August 2016 4,921 5 View
Where can I find the genome and proteome data of pearl millet and other millets? Only Foxtail millet is available in NCBI ftp. Its not their in Gramene too.
20 January 2016 3,128 4 View
1. Which one of these Velvet,SOAP And Abyss is best for a plant genome assembly? 2. correct or feasible to split the data for assembly and then merge/ combine the results of separate files at the...
17 October 2015 7,612 5 View
I want to locate/ map/ mark only a particular gene family on chromosomes of a plant and get a graphical representation. 1. How can I use the bioMart in EnsemblPlants? 2. How can I use it for...
15 October 2015 2,338 4 View
Please, tell me about the techniques and software involved in designing these and how should I design my experiment?
29 December 2014 1,977 5 View
1.If I have all the four species/samples with me i.e. dsDNA, ssDNA, dsRNA and ssRNA, how can I identify and distinguish one from the other? (using spectrophotometer and running them on gel) 2. If...
17 September 2012 4,635 9 View
I have expression analysis data of 7 genes of two different genotypes and of 24 different tissues, can any one tell me about 1. Independent component analysis (ICA) & Principal component...
15 July 2012 5,334 5 View
while using "Nanodrop" for quantification of cDNA, one should quantify it as double-stranded DNA or single-stranded DNA?? I am using Fermentas kit for first standsynthesis of cDNA from total RNA...
30 December 2011 4,433 28 View
