Nazmiara Sabnam

4 Questions 11 Answers 0 Followers

Questions related from Nazmiara Sabnam

Is Southern blot is required for random integration of my protein localization construct transformed into a fungus by ATMT?

Please suggest me what should be done in case I'm doing random integration of my protein localization construct in a fungus by means of ATMT (Agrobacterium Mediated Transformation) where I don't...

07 July 2016 5,756 4 View

Which anti-plagiarism software is useful???

Please suggest me some anti-plagiarism programs that can be used to check presence of plagiarism.

05 May 2016 7,870 6 View

How can I do real time PCR for checking multiple targets compared to the endogenous control gene?

Hi... I have to check many target gene's expression profile by Real-time PCR. I don't want to do a multiplex real-time PCR in a single tube because I'm using SYBR green. If I set an experiment for...

05 May 2016 7,557 9 View

How can I create a translational GFP fusion of (PTrpC promoter + Gene of interest + eGFP + TTrpC Terminator) without frameshifts by overlapping PCR?

Actually I need to make a construct for protein localization and I don't have any idea how to stitch so many fragments together. Can anyone suggest me how to do that starting from primer designing...

08 August 2015 839 4 View