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Questions related from Nazmiara Sabnam
Please suggest me what should be done in case I'm doing random integration of my protein localization construct in a fungus by means of ATMT (Agrobacterium Mediated Transformation) where I don't...
23 July 2016 4,025 4 View
Hi... I have to check many target gene's expression profile by Real-time PCR. I don't want to do a multiplex real-time PCR in a single tube because I'm using SYBR green. If I set an experiment for...
31 May 2016 4,961 8 View
Hi, I'm isolating fungal spores from (8 day old) 10 OMA plates and dissolving in 2 ml water and placing 50 ul drops on the gel bond sheet and incubating till forms appressoria. For harvesting the...
05 May 2016 1,928 4 View
Actually I need to make a construct for protein localization and I don't have any idea how to stitch so many fragments together. Can anyone suggest me how to do that starting from primer designing...
18 August 2015 6,723 4 View
Hi, If anybody can help me with the sequence of pGPTV-BAR vector. I need the sequence of BAR gene or Bialaphos gene. I couldn't find the sequence on ADDGENE. Please help me!!!
26 July 2015 1,609 3 View
I'm doing Southern Blot using Amersham Hybond N+ & ECL detection kit. I am getting a very faint specific band in mutant but not in positive control. I extended the exposure time from 1 hour to...
14 November 2014 8,320 4 View