Nazmiara Sabnam

4 Questions 7 Answers 0 Followers

Questions related from Nazmiara Sabnam

How can I do real time PCR for checking multiple targets compared to the endogenous control gene?

Hi... I have to check many target gene's expression profile by Real-time PCR. I don't want to do a multiplex real-time PCR in a single tube because I'm using SYBR green. If I set an experiment for...

31 May 2016 4,900 8 View

How to isolate total RNA from appressoria germinated on gelbond?

Hi, I'm isolating fungal spores from (8 day old) 10 OMA plates and dissolving in 2 ml water and placing 50 ul drops on the gel bond sheet and incubating till forms appressoria. For harvesting the...

05 May 2016 1,884 4 View

How can I create a translational GFP fusion of (PTrpC promoter + Gene of interest + eGFP + TTrpC Terminator) without frameshifts by overlapping PCR?

Actually I need to make a construct for protein localization and I don't have any idea how to stitch so many fragments together. Can anyone suggest me how to do that starting from primer designing...

18 August 2015 6,672 4 View

Can anybody give me the sequence of pGPTV-BAR vector?

Hi, If anybody can help me with the sequence of pGPTV-BAR vector. I need the sequence of BAR gene or Bialaphos gene. I couldn't find the sequence on ADDGENE. Please help me!!!

26 July 2015 1,459 3 View