Nazmiara Sabnam

6 Questions 11 Answers 0 Followers

Questions related from Nazmiara Sabnam

Is Southern blot is required for random integration of my protein localization construct transformed into a fungus by ATMT?

Please suggest me what should be done in case I'm doing random integration of my protein localization construct in a fungus by means of ATMT (Agrobacterium Mediated Transformation) where I don't...

23 July 2016 4,033 4 View

How can I do real time PCR for checking multiple targets compared to the endogenous control gene?

Hi... I have to check many target gene's expression profile by Real-time PCR. I don't want to do a multiplex real-time PCR in a single tube because I'm using SYBR green. If I set an experiment for...

31 May 2016 4,969 8 View

How to isolate total RNA from appressoria germinated on gelbond?

Hi, I'm isolating fungal spores from (8 day old) 10 OMA plates and dissolving in 2 ml water and placing 50 ul drops on the gel bond sheet and incubating till forms appressoria. For harvesting the...

05 May 2016 1,937 4 View

How can I create a translational GFP fusion of (PTrpC promoter + Gene of interest + eGFP + TTrpC Terminator) without frameshifts by overlapping PCR?

Actually I need to make a construct for protein localization and I don't have any idea how to stitch so many fragments together. Can anyone suggest me how to do that starting from primer designing...

18 August 2015 6,731 4 View

Can anybody give me the sequence of pGPTV-BAR vector?

Hi, If anybody can help me with the sequence of pGPTV-BAR vector. I need the sequence of BAR gene or Bialaphos gene. I couldn't find the sequence on ADDGENE. Please help me!!!

26 July 2015 1,621 3 View

Why am I getting a very faint specific band in the mutant but not in the positive control in Southern blot?

I'm doing Southern Blot using Amersham Hybond N+ & ECL detection kit. I am getting a very faint specific band in mutant but not in positive control. I extended the exposure time from 1 hour to...

14 November 2014 8,323 4 View