Nazilah Satar

4 Questions 8 Answers 0 Followers

Questions related from Nazilah Satar

How to collect cancer cell line condition medium (secretome)?

Hi, Has anyone here has done condition medium from cancer cell line and run the samples for LCMS? I have done it by culturing the cells in the medium and then starving(serum-free to avoid the...

03 March 2017 2,934 1 View

IC 50

Hi , I'm just curious why do we use IC50? not IC40 or IC90.? Are there any specific reasons?

12 December 2016 6,291 0 View

Difference between mass spectometry and LCMS?

HI, What is the differences between mass spectometry and LCMS. I knew that both identify protein and im concern tha LCMS is more sensitive for detecting the protein from sampels. But could anyone...

04 April 2016 618 5 View

What other method can be used to evaluate secretome other than 2D gel?

I am working on evaluating cancerous niches using secretome media and will further with 2D-gel. Do we have any others methods to evaluate secretome other than 2D-gel or LCMS? Beside, is there any...

12 December 2015 1,424 3 View