Nazilah Satar

5 Questions 11 Answers 0 Followers

Questions related from Nazilah Satar

How to collect cancer cell line condition medium (secretome)?

Hi, Has anyone here has done condition medium from cancer cell line and run the samples for LCMS? I have done it by culturing the cells in the medium and then starving(serum-free to avoid the...

01 March 2017 4,800 1 View

Problem in transducing A549 cell line using pTRIPZ lentiviral inducible system?

Hi everyone, I alreadydone co-transfection of Dharmacon Trans-lentiviral packaging mix and shRNA transfer vector into HEK 293T. The transfection efficiency was 90-95%. I proceeded with virus...

25 April 2016 4,355 2 View

How to calculate a right dose for in vivo study?

Hi, I am going to start the in vivo study. However, i have no clue on how to administered the doses of my agent (curcumin and drugs) in to the mice. What and how is the calculation to determine...

29 February 2016 3,906 8 View

How can I get a spot for 2D using secretome?

Currently, i'm working on secretome study and i use 2D to evaluate the protein. Unfortunately, i've got no spots after staining the gel with commasive blue. My concentration was 400ug and i used...

03 December 2015 7,595 10 View

How do I increase protein concentration of condition media?

I work with protein secretion using condition media. I've collect and concentrate the condition media using 10kdA Amicon Ultra Filter (Milipore) and proceed with SDS page at concentration 4ug/ul...

18 November 2015 6,499 8 View