What is the main problems if the middle protein band does not found in nitrocelluse membrane except higher and lower protein band of Western Blot?

I did the western blot many times using my mice liver tissue homogenate. But i did not got my desire protein band such as MT, Nrf2, HO-1 and even beta actin on nitrocelluse membrane whereas i got...

07 July 2016 9,871 6 View