Nawraj Rummun

3 Questions 4 Answers 0 Followers

Questions related from Nawraj Rummun

Measurement of Nitric oxide from LPS stimulated Raw264.7 cells?

HI all, I am trying to assess the antiinflammatory effect of herbal extracts by measuring the NO release in LPS stimulated Raw cells. I am seeding 200 ul of 5x 104 cells in a 96 well plate on day...

11 August 2025 5,939 2 View

Do we express results of activity guided fractionation for isolation of bioactive compounds in terms of lyophilized weight or air dry weight?

22.3 g fresh leaf was air dried to constant mass resulting in 10 g air dry weight. The air dry leaf were pulverized and extracted exhaustively with 80 % methanol. The macerate was concentrated and...

23 August 2016 5,605 3 View

Can we quantify the terpenoid content of a herbal extract using spectrometric/ colorimetric method only without the use of HPLC?

Similar to approaches used for quantification of total phenolic content. May someone please suggest research article with protocols for the same, which I may refer to. Thanks

23 September 2014 373 4 View