Nawaj Shaikh

2 Questions 9 Answers 0 Followers

Questions related from Nawaj Shaikh

What should be optimum concentration of DMSO for in cell culture (Suspension celllines)?

I am dissolving some cell culture ligands in 100% DMSO to make stock solutions of 5ug/ 10ul of DMSO. Im then adding 20 ul of this stock to 1 ml of media+cells in 12 well plates to make a final...

11 November 2015 8,350 8 View

Can anyone recommend a protocol to harvest macrophages derived from THP-1 cells, by scrapping method?

I am interested to see the interferon secretion and RNA extraction from these harvested macrophages to see its further in-vitro action on HIV infected cells. Chemical method may interfere in...

10 October 2014 4,605 10 View