Navodya Silva

5 Questions 2 Answers 0 Followers

Questions related from Navodya Silva

Can cells deprived of CO2 be revived and be used for later assay?

Something went wrong with our cell incubator over the weekend so to my knowledge the cells inside were deprived of a sufficient level of Carbon Dioxide for about 24 hours. The photo attached shows...

28 November 2023 1,914 1 View

Colony formation Assay. Why are the NTERA cells in my colonies dying?

I am doing a colony formation assay on 96 well plates. Each well has 150 NTERA-D2 cells. The incubation times for this assay are 4 days, 7 days, 10 days, and 14 days. I replenish the media every...

02 October 2023 3,491 2 View

Colony Formation Assay: When should I replenish media?

I have a colony formation assay. I am seeding cells in a 96-well plate. The cell line is NTERA, 150 cells per well. I am seeding the cells with DMEM fortified with 10% FBS. Each well will have...

20 September 2023 9,083 2 View

Scratch Assay : Small gaps in the Edges of the well. Will this effect the results of the scratch assay?

I have seeded 50,000 NTERA cells per well in a 96-well plate in preparation for a scratch assay. I seeded the cells on the 11th of September. And even after 48 hours (13th of September), some of...

13 September 2023 9,311 1 View

Question about Cell Culture: How can I keep my seeded cells from accumulating in the corners of the well when seeding?

For my research project, I often have to seed cells into cell culture plates for cytotoxicity assays like SRB and MTT. I have noticed that whenever I seed cells into the wells of a 96-well treated...

26 August 2023 8,149 4 View