Navin Ray

4 Questions 10 Answers 0 Followers

Questions related from Navin Ray

Problem in resolving gel interface, Lateral side of the gel has problem. The Top of the gel looks fine sharp. anyonne? : SDS-PAGE, western blotting?

i use the same reagents that my labmates use, but i have this queer problem killing me: after mixing the resolving gel ingredients (d.water, tris, acryl, aps, temed), when i pour the gel to the...

15 February 2018 4,341 3 View

When i pour alcohol (100% ethanol or iso-propanol) to overlay level, it penetrates the resolving gel, is there any solution to this problem?

hello, when i pour alcohol (100% ethanol or iso-propanol) to overlay the resolving gel level, it penetrates the resolving gel, and makes a wavy line, or a trail that seems to pass across the...

18 January 2018 6,923 9 View

Why do the apoptotic markers- Parp, cleaved parp, caspase3, cleaved caspase3 decrease with increases in pro-apoptotic drug dose?

I am treating drug which causes cell death (according to mtt assay), in dose dependent manner, but in western blot same ratio of drug does not show increased activity of cleaved- Caspase3 and...

22 September 2015 7,942 8 View

Is there any significance if the western blot of Akt and P-Akt is decreased with the incresing concn. of apoptotic drug?

i treated apoptosis inducing DRUG in cancer cells in an increasing order, the expression of AKT and p-Akt is found decreasing, How can I explain the DATA ? TIA

15 August 2015 8,864 15 View