Navid Paknejad

3 Questions 6 Answers 0 Followers

Questions related from Navid Paknejad

What substrate combines atomic flatness with good optical properties for simultaneous IF/AFM imaging of fluorescently-labelled DNA?

I am looking to co-localize a phenomenon on DNA via IF and AFM methods. Mica yielded beautiful AFM images, but no IF signal. Glass yielded a great IF image, but was too rough to resolve the DNA...

03 March 2014 6,633 4 View

What are some optimal surfaces for AFM imaging of proteins (IgG, IgM)?

I have experimented with imaging proteins (IgG, IgM) on mica and glass, both with treatments of APTES to generate AP-Mica and a glutaraldehyde treatment to expose reactive aldehyde groups on the...

03 March 2014 3,181 4 View

AFM imaging of live cells in contact mode - protocol, culture surface, surface treatment, tip selection, etc: what are your preferences?

I have been trying to image live endothelial cells (contact mode) with our atomic force microscope (AFM) for the past few weeks. I can image fixed cells perfectly fine, but living cells are much...

05 May 2013 5,813 16 View