Nausheen Jamal

3 Questions 7 Answers 0 Followers

Questions related from Nausheen Jamal

Protein folding: How can I refold proteins from inclusion bodies in a single conformation?

I want to purify a recombinant protein from inclusion bodies. After the re-folding and dialysis step, the protein elutes in two distinct peaks on ion exchange chromatography. First peak has poor...

10 October 2014 7,833 8 View

Why might the band intensity on PAGE not correspond to the absorbance at 280 nm during purification of a recombinant protein?

I am purifying a recombinant protein (expressed as inclusion bodies) on a ion exchange column eluting with a gradient of NaCl. A sharp peak is seen in elution which scores pretty high on...

06 June 2014 4,898 6 View

What is a reliable method for the analysis of sugars / proteoglycans immobilized on agarose resin?

For the preparation of affinity chromatography column, once a ligand (carbohydrate) is coupled to agarose resin what would be a reliable method to check if the coupling reaction went well?

02 February 2014 4,261 0 View