Naushad Moti

5 Questions 15 Answers 0 Followers

Questions related from Naushad Moti

I need help in the adding of Nucleotides to DNA sequence manually. Anyone with experience out there?

Hi All, I am looking to add 3 nucleotides to a position in my DNA sequence which won't disrupt the coding region but introduce a restriction site into it. Is there any way I can add 3 different...

10 October 2014 9,547 6 View

Can anyone help me with ligation that is working but transformation that doesn't ?

Hi Folks, I am cloning my gene of interest using PCR method. I did my PCR, purified the product, restriction digested it, purified again and then ligated it. My uncut is giving me lots of colonies...

08 August 2014 5,066 17 View

Can anyone help me with replacing GFP with RFP?

Hi, I need to replace GFP with other chromophores in my plasmid. I don't have any restriction sites (single) that I can use to excise my gene off and replace it with YFP or CFP or RFP. Any...

07 July 2014 9,953 10 View

Can anyone help with blunt and sticky ends?

I have a plasmid with RE sites that produce blunt and sticky ends. I need to do cloning on this. Is it possible to ligate both ends simultaneously together in one reaction?

06 June 2014 6,579 5 View

Can anyone give any suggestions for gateway cloning?

I am planning to do some gateway cloning and I have all the pENTR/TOPO vectors with attB sites. What I need to know is how to add these attB sites into my vector so I can easily exchange my gene...

06 June 2014 4,401 5 View