Is it possible to have a leaky expression of a mutant protein while the wild-type protein's is inducible?

i did a site-directed mutagenesis of HBx gen in pET-16b plasmid to produce HBx T118N. The HBx wild-type overproduction in e.coli BL21(DE3) required IPTG 0,1 mM. On the other hand, HBx T118N could...

16 May 2018 585 3 View