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Questions related from Nathanael Quake
Hi all, May I have some suggested reasons why I am obtaining amplification curves (in pink) with unusual shapes (particularly near the baseline) and plateaus of various heights? This is a...
12 December 2019 8,909 4 View
Hi all, I am currently studying microbiology. I was taught that E.coli, like all members of Enterobacteriaceae, is oxidase negative. However, isn't E.coli capable of aerobic respiration, hence...
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Dear all, I am currently designing an ARMS-qPCR assay to detect mutant alleles at very low levels while diluted with a high number of wild type alleles in the same solution. I first did a...
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Dear all, Currently, I am using plasmids as controls in qPCR. These plasmids were freshly extracted from bacteria using a maxiprep. I diluted the plasmids to 100, 000 copies used them as templates...
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Dear all, I have no experience with the QIACube. However, I wish to automate DNA extraction for a variable number of samples. The machine often advertised to work with 12 samples. Is it possible...
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Dear all, I am designing a qPCR assay with a Taqman probe and primers. I want the amplicon to be as small as possible. Hence, I would like to know if there is a recommended minimum number of...
10 October 2016 6,343 3 View
Dear all, I amplified a sequence from a plasmid template. The amplicons will serve as templates for IVT. The amplicon is 500bp in size, while the plasmid is about 2000bp in size. I do not wish to...
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Hi all, I am currently using qPCR extensively, and I have some questions regarding relative quantification and PCR efficiency. If I use the Livak method for relative quantification, does it...
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