Nathan Rockwell

2 Questions 2 Answers 0 Followers

Questions related from Nathan Rockwell

Has anyone ever used Sf9 cells from Novagen (Millipore Sigma)?

They are 1/3 the price of ATCC and Thermo, but I can't find any huge difference. Is there a difference in growth or transfection efficiency that would account for the difference in cost....

01 January 2018 10,011 1 View

My media is turning yellow, but is mycoplasma negative and the incubator is fine. What could it be?

I'm culturing primary mouse astrocytes with mutant p53 in DMEM/F12 + 10% FBS. After a few days in culture, the media starts to rapidly turn yellow and the cells die. The cells are mycoplasma...

04 April 2017 7,415 7 View