Nathan Ahlgrim

4 Questions 5 Answers 0 Followers

Questions related from Nathan Ahlgrim

Does transfection occur when you infuse virus into the ventricle?

I am trying to develop a rat model for optogenetic stimulation of the basolateral amygdala complex. Twice now, I have infused a viral vector carrying a channelrhodopsin variant under a CaMKIIa...

11 November 2016 1,224 6 View

How can I label brain hemispheres for free-floating histology?

I am running immunohistochemistry on rat brain tissue, and the sections will be stained while free-floating. However, we need to reliably know the left from right hemisphere. We cannot take a...

04 April 2015 5,115 9 View

Is anyone familiar with Fixing brain tissue before freezing?

I was recently told by the director of our pathology core that brain tissue must not be perfused if it is to be frozen (to be later sectioned via cryostat). She said it would lead to shattering of...

04 April 2015 4,576 7 View

Is there a way to get a validation for the microdissection of a rat brain?

I am trying to collect the hippocampus, prefrontal cortex and amygdala from adolescent rat brains (unfixed). Is there a way I could validate the accuracy of the dissection to rule out...

07 July 2014 3,583 3 View