Natasha Naidoo

2 Questions 5 Answers 0 Followers

Questions related from Natasha Naidoo

Why are my HSA and BSA running lower than the expected molecular weight on SDS-PAGE?

I ran out my BSA and HSA on SDS-PAGE (6% gel). I have them in normal 20mM Tris buffer at pH 6.5. I used the New England Biolabs blue protein standard. Both the HSA (Sigma) and BSA (Pierce) are...

03 March 2016 5,539 6 View

Can I use HSA as a carrier protein if I am screening human sera?

I am doing an ELISA and my peptide will be conjugated to HSA. I am screening human serum for the antibody of interest. Will the HSA react with the human serum? If so, I need a suitable alternative...

07 July 2015 4,248 4 View