Natasha Naidoo

2 Questions 2 Answers 0 Followers

Questions related from Natasha Naidoo

Can I use HSA as a carrier protein if I am screening human sera?

I am doing an ELISA and my peptide will be conjugated to HSA. I am screening human serum for the antibody of interest. Will the HSA react with the human serum? If so, I need a suitable alternative...

14 July 2015 5,515 4 View

Why is my peptide cleaving off GST tag upon expression and purification?

I have expressed a 4kDa peptide on a GST tag using a pGEX vector. My peptide cleaves off the GST tag and when I run the gel after purification on glutathione agarose, I see two bands corresponding...

18 June 2015 6,013 4 View