Natasha Naidoo

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Questions related from Natasha Naidoo

How do you determine assembly factors of pili that dont have all components in an operon

Mycobacterial curli pili dont have an operon of pili secretion and assembly factors and chaperones. Its a very different picture from other pili types that usually consist of the components of a...

19 June 2017 3,401 2 View

Can I use HSA as a carrier protein if I am screening human sera?

I am doing an ELISA and my peptide will be conjugated to HSA. I am screening human serum for the antibody of interest. Will the HSA react with the human serum? If so, I need a suitable alternative...

14 July 2015 5,550 4 View

Why is my peptide cleaving off GST tag upon expression and purification?

I have expressed a 4kDa peptide on a GST tag using a pGEX vector. My peptide cleaves off the GST tag and when I run the gel after purification on glutathione agarose, I see two bands corresponding...

18 June 2015 6,055 4 View