Natascha Techen

4 Questions 16 Answers 0 Followers

Questions related from Natascha Techen

How to improve antigene amino acid sequence to make good antibodies?

I want to detect a protein that is present in full length or truncated. I mostly want to detect the truncated version, about 14 AA from the amino-terminus but the antigenicity of the peptide is...

09 September 2017 2,608 6 View

How to covalently bind oligonucleotides to magnetic beads?

I want to use oligonucleotides to pull out DNA fragments from a library. I don't want to use biotin/streptavidin magnetic beads because need to work at temperatures above 45°C (streptavidin/biotin...

10 October 2016 5,382 22 View

Preparing stock cultures for protein expression in BL21DE3 RIPL cells

I was wondering if you have tried this protocol with BL21DE3 RIPL cells. Is the protein expression from the stock culture as good as from fresh ones? Make a Stock Culture The way to work with...

03 March 2013 6,535 2 View

How long are M-280 Dynabeads (Streptavidin) stable?

I have Dynabeads that are 5 years old now and was wondering if they are still good to use. Any ideas?

11 November 2012 7,178 7 View