Nataliya Slater

3 Questions 5 Answers 0 Followers

Questions related from Nataliya Slater

Protein not running on Native PAGE - why and how can I fix it?

I am trying to confirm whether my recombinant protein of interest is a multimer. The only technology I have access to is native PAGE. What I know is that the monomer is a 43 kDa cytosolic protein....

04 March 2025 3,344 7 View

Has anyone seen such a B cell ELISPOT result before?

I have carried out an ELISPOT with human B cells that were stimulated with R848 (TLR7/8 antagonist) and IL-2 for 7 days prior to being added to the ELISPOT plate coated with goat anti-human IgG...

09 February 2023 2,636 1 View

How can I enzymatically cleave a his-tagged protein off magnetic beads?

For my experiment I have immobilised a his tagged protein on Dynabeads his-tag isolation and pulldown magnetic beads that will be used to pull out specific cells from PBMCs. I need to...

25 July 2022 3,306 5 View