Natalie Shepherd

3 Questions 8 Answers 0 Followers

Questions related from Natalie Shepherd

What is the maximum volume of DNA you can concentrate using the NaOAc method?

I need to concentrate 10 mL of DNA down to 100 uL as I need a very high DNA concentration for an NGS application. Has anyone used the NaOAc/EtOH precipitation method to concentrate from such a...

03 March 2015 4,414 9 View

Ganglioside GT1b binding assay

I am trying to assess binding activity of a His-tagged recombinant to ganglioside GT1b via a sandwich assay (ELISA). I have performed the experiment numerous times with 2 different gangliosides...

02 February 2012 3,114 5 View

Dialysis D-Tube™ Dialyzers (Merck) - anyone have experience?

I have to do some dialysis on very small volumes and just found out about the D-Tube™ Dialyzers from Merck. They seem great in principle. Does anyone have experience with these and have any...

07 July 2011 5,999 3 View