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Questions related from Natalie Mikita
I am trying to do some recombineering to knock out a gene in BL21 DE3 that is interfering with my POI purification. I ordered a plasmid with the lambda red genes in it from addgene which is also...
06 July 2020 6,685 0 View
I am trying to purify a protein out of a BL21 E. coli. I have inserted a pET24b plasmid with the gene into the BL21. The problem is that I can't separate it from an essential homolog in the...
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I am working with a serine protease. After purification, I ran a degradation assay with casein, and it appeared to work great twice in a row (first picture representative of both attempts, first...
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