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Hi I am using Homologous Recombination to make change in my virus DNA with the plasmid construct which I have already prepared . I have heard this method is really hard and time consuming to be...
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I am trying to insert my fragment in pET21 plasmid, but if I do not reverse pET21 plasmid in CLC or other software, the direction of restriction sites will be disrupted. I was wondering if anyone...
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