Narjis Fatima

2 Questions 4 Answers 0 Followers

Questions related from Narjis Fatima

CID MS from peptides enriched using invitrogen protein G dynabeads ?

Hi , I am using invitrogen protein G dynabeads to enrich proteins, but the yield I am getting is around 20 ug. I need to do CID MS after pull downs, and require atleast 100ug proteins in initial...

07 July 2019 3,867 2 View

What is the best way to quantify the proteins in western blot?

I have used Image Studio lite software, and it gives the respective intensities of the proteins. Can anyone guide me how to convert those intensities in fold change? I am working with wet transfer...

06 June 2016 243 6 View