Narin Nard Carmel Neiderman

4 Questions 4 Answers 0 Followers

Questions related from Narin Nard Carmel Neiderman

Does any of you have protocols for tissue culture dosage/usage of the following drugs: Rifampicin, Iopanoic acid Hexetidine?

I only find online the human dosage and i will be happy to find a good protocol to focus my calibrations... thanks!

28 October 2017 1,850 2 View

OTC high variable detectin in RT PCR- why?

Hey, I am doing RT PCR with both TAQ and SYBER primers. My normalizing genes are great, unify, and perfect. However OTC, even in HepG2 cell line, is not unify and raises in the same technical...

23 November 2016 6,278 3 View

How can I use sonication for nuclei membrane removal?

i fractionated cell culture via a kit but i do not want to use the kit further after cytoplasm removal because i am afraid the detergents for nuclear extraction might ruin protein protein...

29 February 2016 2,128 4 View

why do I get a weak DAPI staining?

Hey! I have a stock of 1:1000 DAPI. I used for 50 microliter of sample 1-2 microliter of DAPI and the stain is still very weak. I incubated DAPI with conjugated ab for 20 min, but even samples...

12 January 2016 8,887 4 View