Nareshwaran Gnanasegaran

4 Questions 18 Answers 0 Followers

Questions related from Nareshwaran Gnanasegaran

Has anyone used PKH26 for cell tagging purposes?

We wanted to study the cell bio-distribution in our animal models and we have used PKH26 in order to tag our cells. The cells can be observed in vitro using a fluorescent microscope (using...

12 December 2014 1,700 2 View

Could anyone share established protocol on how to culture neuronal cells from rat's brain?

I would like to culture rat's brain cells for my upcoming experiments, however, I'm not sure as to which protocol should I use for this purpose. Any suggestions? Thanking in advance. Regards, Naresh.

12 December 2014 7,806 5 View

Has anyone used Fx Cycle (Life Technologies) for cell cycle analysis?

I have performed cell cycle analysis with this reagent following the protocol provided, however the output was not as expected. I used normal cells without any treatment so the typical G0/G1, S,...

06 June 2014 5,827 7 View

Can I store my cultured cells for longer period just by fixing them with PFA?

I have cells but the Flow Cytometry machine was not working and will be sent for servicing. So, I was thinking whether can I store PFA-fixed cells in certain condition till the machine is back....

01 January 2014 8,473 7 View