Can someone tell me what's the best method to purify and quantify Leishmania amastigotes from spleen and or liver?

HI; May any one tell me what's the best method to purify and quantify Leishmania amastigotes from spleen and or liver. We've tried parasite burden by limit dilution and it didn't work well.thank's

29 January 2015 4,717 3 View