I forgot to add CACC sequence in 1 of the forward primer I designed to amplify insert for pENTR D-TOPO cloning. Is there a way to rectify this?

To me it seems impossible to proceed for pENTR D-TOPO cloning without the insert having CACC over hangs, I would like to know what others think about this.

07 July 2018 305 4 View