Nandita Keshavan

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Questions related from Nandita Keshavan

I have been trying to express constructs in E coli for protein expression. I have seen a low yield in insoluble protein pellet. What may cause this?

I normally pellet 1 ml of the expression culture to check expression. I then use a detergent lysozyme lysis method which works on our positive control. Other than errors in cloning, is there any...

05 May 2013 2,742 4 View

Is it necessary to add a stabilising agent eg glycerol or sucrose when freezing a protein?

We don't know whether the agent will interfere with a translation assay we want to do. Would proteins usually get dentaured if they are snap frozen in a buffer without stabilisation?

11 November 2012 1,909 23 View