Does overnight resting of PBMCs help in better detection of IFNg, TNFa and IL2?

I want to detect IFNg, TNFa/IL-2 and CD107a in NK and T cells. I am going to stimulate them with different stimuli (PMA, Anti-CD16, K562, etc). Before I start my optimization I wanted to know...

21 May 2014 889 4 View