How to troubleshoot in primer designing for a gene when we have to select 30 bp (minimum) to get Tm close to 60 degree?

I am trying to design a primer for a specific gene and the Tm is coming near to 60 degrees when I select a minimum of 30-35 bp, for forward as well as for reverse primer. As the template is too...

07 July 2023 4,732 4 View